borosilicate glass capillary recording electrode  (Sutter Instrument Company)

Journal: eNeuro

Article Title: Timing of Morphine Administration Differentially Alters Paraventricular Thalamic Neuron Activity

doi: 10.1523/ENEURO.0377-19.2019

Figure Lengend Snippet: Morphine administration during the light cycle increases the number of spontaneously firing PVT neurons. A1 , ZT timeline showing times in which acute brain slices were prepared. Slices were prepared at either ZT2 (light) or ZT14 (dark). A2 , Coronal mouse brain slice showing an electrophysiological recording in the PVT (dashed shape outlines the recording electrode that is patched onto a PVT neuron). B , Representative traces showing the firing of PVT neurons 24 h following saline or morphine administration during light or dark cycles. C , Quantification of silent or spontaneously firing PVT neurons following repeated saline or morphine treatment. D , Summary showing the overall firing frequency of PVT neurons 24 h following repeated saline (Sal) or morphine (Mor) administration ( F (3,52) = 5.52, p = 0.002, one-way ANOVA); * p < 0.05. Scale bars: 40 mV, 2 s.

Article Snippet: For recordings of spontaneously firing neurons , recording electrodes [2–5 MΩ; borosilicate glass capillaries (WPI #1B150F-4) pulled on a horizontal puller from Sutter Instruments (model P-97)] were filled with a potassium-based internal solution containing the following: 130 mM KMeSO 3 , 10 mM KCl, 10 mM HEPES, 0.4 mM EGTA, 2 mM MgCl 2 -6H 2 0, 3 mM Mg-ATP, and 0.5 mM Na-GTP, pH 7.2–7.4.

Techniques: Slice Preparation, Saline